Effects of a SARS-associated coronavirus vaccine in monkeys
Identifieur interne : 006024 ( Main/Exploration ); précédent : 006023; suivant : 006025Effects of a SARS-associated coronavirus vaccine in monkeys
Auteurs : Wentao Gao [États-Unis] ; Azaibi Tamin [États-Unis] ; Adam Soloff [États-Unis] ; Leonardo D'Aiuto [États-Unis] ; Edward Nwanegbo [États-Unis] ; Paul D. Robbins [États-Unis] ; William J. Bellini [États-Unis] ; Simon Barratt-Boyes [États-Unis] ; Andrea Gambotto [États-Unis]Source :
- Lancet (London, England) [ 0140-6736 ] ; 2003.
Descripteurs français
- KwdFr :
- Adenoviridae (immunologie), Animaux, Coronavirus (immunologie), Humains, Lymphocytes T (immunologie), Macaca mulatta, Maladies des singes (), Maladies des singes (virologie), Modèles animaux de maladie humaine, Production d'anticorps (immunologie), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (immunologie), Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Technique de Western, Vaccination (), Vaccins antiviraux (immunologie), Vaccins antiviraux (usage thérapeutique), Virus du SRAS (immunologie).
- MESH :
- immunologie : Adenoviridae, Coronavirus, Lymphocytes T, Production d'anticorps, Protéines de l'enveloppe virale, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- usage thérapeutique : Vaccins antiviraux.
- virologie : Maladies des singes.
- Animaux, Humains, Macaca mulatta, Maladies des singes, Modèles animaux de maladie humaine, Syndrome respiratoire aigu sévère, Technique de Western, Vaccination.
English descriptors
- KwdEn :
- Adenoviridae (immunology), Animals, Antibody Formation (immunology), Blotting, Western, Coronavirus (immunology), Disease Models, Animal, Humans, Macaca mulatta, Monkey Diseases (prevention & control), Monkey Diseases (virology), Nucleocapsid Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), T-Lymphocytes (immunology), Vaccination (methods), Viral Envelope Proteins (immunology), Viral Vaccines (immunology), Viral Vaccines (therapeutic use).
- MESH :
- chemical , immunology : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Vaccines.
- immunology : Adenoviridae, Antibody Formation, Coronavirus, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- methods : Vaccination.
- prevention & control : Monkey Diseases, Severe Acute Respiratory Syndrome.
- chemical , therapeutic use : Viral Vaccines.
- virology : Monkey Diseases.
- Animals, Blotting, Western, Disease Models, Animal, Humans, Macaca mulatta.
Abstract
The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.
Url:
DOI: 10.1016/S0140-6736(03)14962-8
PubMed: 14667748
PubMed Central: 7112457
Affiliations:
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xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff5">National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Soloff, Adam" sort="Soloff, Adam" uniqKey="Soloff A" first="Adam" last="Soloff">Adam Soloff</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department 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Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Nwanegbo, Edward" sort="Nwanegbo, Edward" uniqKey="Nwanegbo E" first="Edward" last="Nwanegbo">Edward Nwanegbo</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Robbins, Paul D" sort="Robbins, Paul D" uniqKey="Robbins P" first="Paul D" last="Robbins">Paul D. Robbins</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff5">National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Barratt Boyes, Simon" sort="Barratt Boyes, Simon" uniqKey="Barratt Boyes S" first="Simon" last="Barratt-Boyes">Simon Barratt-Boyes</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4">Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Gambotto, Andrea" sort="Gambotto, Andrea" uniqKey="Gambotto A" first="Andrea" last="Gambotto">Andrea Gambotto</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff3">Department of Medicine, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author></analytic><series><title level="j">Lancet (London, England)</title><idno type="ISSN">0140-6736</idno><idno type="eISSN">1474-547X</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (immunology)</term><term>Animals</term><term>Antibody Formation (immunology)</term><term>Blotting, Western</term><term>Coronavirus (immunology)</term><term>Disease Models, Animal</term><term>Humans</term><term>Macaca mulatta</term><term>Monkey Diseases (prevention & control)</term><term>Monkey Diseases (virology)</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>T-Lymphocytes (immunology)</term><term>Vaccination (methods)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term><term>Viral Vaccines (therapeutic use)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (immunologie)</term><term>Animaux</term><term>Coronavirus (immunologie)</term><term>Humains</term><term>Lymphocytes T (immunologie)</term><term>Macaca mulatta</term><term>Maladies des singes ()</term><term>Maladies des singes (virologie)</term><term>Modèles animaux de maladie humaine</term><term>Production d'anticorps (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Technique de Western</term><term>Vaccination ()</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins antiviraux (usage thérapeutique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Adenoviridae</term><term>Coronavirus</term><term>Lymphocytes T</term><term>Production d'anticorps</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Adenoviridae</term><term>Antibody Formation</term><term>Coronavirus</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Monkey Diseases</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des singes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Monkey Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Humans</term><term>Macaca mulatta</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Macaca mulatta</term><term>Maladies des singes</term><term>Modèles animaux de maladie humaine</term><term>Syndrome respiratoire aigu sévère</term><term>Technique de Western</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author><author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name></author><author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anton, Im" uniqKey="Anton I">IM Anton</name></author><author><name sortKey="Gonzalez, S" uniqKey="Gonzalez S">S Gonzalez</name></author><author><name sortKey="Bullido, Mj" uniqKey="Bullido M">MJ Bullido</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Weiss, Rc" uniqKey="Weiss R">RC Weiss</name></author><author><name sortKey="Scott, W" uniqKey="Scott W">W Scott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T Kuiken</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M Schutten</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Gao, Wentao" sort="Gao, Wentao" uniqKey="Gao W" first="Wentao" last="Gao">Wentao Gao</name></region><name sortKey="Barratt Boyes, Simon" sort="Barratt Boyes, Simon" uniqKey="Barratt Boyes S" first="Simon" last="Barratt-Boyes">Simon Barratt-Boyes</name><name sortKey="Barratt Boyes, Simon" sort="Barratt Boyes, Simon" uniqKey="Barratt Boyes S" first="Simon" last="Barratt-Boyes">Simon Barratt-Boyes</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name><name sortKey="D Aiuto, Leonardo" sort="D Aiuto, Leonardo" uniqKey="D Aiuto L" first="Leonardo" last="D'Aiuto">Leonardo D'Aiuto</name><name sortKey="Gambotto, Andrea" sort="Gambotto, Andrea" uniqKey="Gambotto A" first="Andrea" last="Gambotto">Andrea Gambotto</name><name sortKey="Gambotto, Andrea" sort="Gambotto, Andrea" uniqKey="Gambotto A" first="Andrea" last="Gambotto">Andrea Gambotto</name><name sortKey="Gambotto, Andrea" sort="Gambotto, Andrea" uniqKey="Gambotto A" first="Andrea" last="Gambotto">Andrea Gambotto</name><name sortKey="Nwanegbo, Edward" sort="Nwanegbo, Edward" uniqKey="Nwanegbo E" first="Edward" last="Nwanegbo">Edward Nwanegbo</name><name sortKey="Robbins, Paul D" sort="Robbins, Paul D" uniqKey="Robbins P" first="Paul D" last="Robbins">Paul D. Robbins</name><name sortKey="Soloff, Adam" sort="Soloff, Adam" uniqKey="Soloff A" first="Adam" last="Soloff">Adam Soloff</name><name sortKey="Soloff, Adam" sort="Soloff, Adam" uniqKey="Soloff A" first="Adam" last="Soloff">Adam Soloff</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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